High efficiency callus induction and plant regeneration in petiole culture of four poplar genotypes.
Identifieur interne : 004A81 ( Main/Exploration ); précédent : 004A80; suivant : 004A82High efficiency callus induction and plant regeneration in petiole culture of four poplar genotypes.
Auteurs : M A Jafari [Hongrie] ; J. Kiss ; J. Gergácz ; L E HeszkySource :
- Acta biologica Hungarica [ 0236-5383 ] ; 1995.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Arbres, Plantes, Régénération.
- physiologie : Arbres.
- Génotype, Milieux de culture, Phénomènes physiologiques des plantes, Protoplastes, Transformation génétique.
English descriptors
- KwdEn :
- MESH :
- chemical : Culture Media.
- genetics : Plants, Regeneration, Trees.
- physiology : Trees.
- Genotype, Plant Physiological Phenomena, Protoplasts, Transformation, Genetic.
Abstract
In order to develop a simple and feasible approach to achieve high frequency plant regeneration for protoplast isolation and transformation experiments, a method was elaborated by using a new type of explant (petiole segments) for the four Populus nigra genotypes. Callus initiation from the petioles took place on N6 medium containing 2,4-D (0.1-1 mg/l). The highest rate of callus initiation (100%) was achieved when the basic medium was supplemented with 0.5 mg/l of 2,4-D, in all tested genotypes. For shoot regeneration, calli were transferred to MS and WPM medium supplemented with BA (1.0-2.5 mg/l) and NAA (0.2 mg/l). Multiple shoot regeneration was observed in each shoot induction medium. The highest rate of shoot regeneration (6.83 shoot/callus) was observed on MS medium containing 2.5 mg/l BA and 0.2 mg/l NAA. The results showed highly significant differences between the media. There was no significant difference between the genotypes and genotype x medium interaction.
PubMed: 8714763
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Gergácz</name></author><author><name sortKey="Heszky, L E" sort="Heszky, L E" uniqKey="Heszky L" first="L E" last="Heszky">L E Heszky</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1995">1995</date><idno type="RBID">pubmed:8714763</idno><idno type="pmid">8714763</idno><idno type="wicri:Area/Main/Corpus">004B03</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004B03</idno><idno type="wicri:Area/Main/Curation">004B03</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004B03</idno><idno type="wicri:Area/Main/Exploration">004B03</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">High efficiency callus induction and plant regeneration in petiole culture of four poplar genotypes.</title><author><name sortKey="Jafari, M A" sort="Jafari, M A" uniqKey="Jafari M" first="M A" last="Jafari">M A Jafari</name><affiliation wicri:level="1"><nlm:affiliation>Gödöllõ University of Agricultural Sciences, Department of Genetics and Plant Breeding, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>Gödöllõ University of Agricultural Sciences, Department of Genetics and Plant Breeding</wicri:regionArea><wicri:noRegion>Department of Genetics and Plant Breeding</wicri:noRegion></affiliation></author><author><name sortKey="Kiss, J" sort="Kiss, J" uniqKey="Kiss J" first="J" last="Kiss">J. 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Callus initiation from the petioles took place on N6 medium containing 2,4-D (0.1-1 mg/l). The highest rate of callus initiation (100%) was achieved when the basic medium was supplemented with 0.5 mg/l of 2,4-D, in all tested genotypes. For shoot regeneration, calli were transferred to MS and WPM medium supplemented with BA (1.0-2.5 mg/l) and NAA (0.2 mg/l). Multiple shoot regeneration was observed in each shoot induction medium. The highest rate of shoot regeneration (6.83 shoot/callus) was observed on MS medium containing 2.5 mg/l BA and 0.2 mg/l NAA. The results showed highly significant differences between the media. There was no significant difference between the genotypes and genotype x medium interaction.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8714763</PMID><DateCompleted><Year>1996</Year><Month>10</Month><Day>02</Day></DateCompleted><DateRevised><Year>2008</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0236-5383</ISSN><JournalIssue CitedMedium="Print"><Volume>46</Volume><Issue>1</Issue><PubDate><Year>1995</Year></PubDate></JournalIssue><Title>Acta biologica Hungarica</Title><ISOAbbreviation>Acta Biol Hung</ISOAbbreviation></Journal><ArticleTitle>High efficiency callus induction and plant regeneration in petiole culture of four poplar genotypes.</ArticleTitle><Pagination><MedlinePgn>51-9</MedlinePgn></Pagination><Abstract><AbstractText>In order to develop a simple and feasible approach to achieve high frequency plant regeneration for protoplast isolation and transformation experiments, a method was elaborated by using a new type of explant (petiole segments) for the four Populus nigra genotypes. Callus initiation from the petioles took place on N6 medium containing 2,4-D (0.1-1 mg/l). The highest rate of callus initiation (100%) was achieved when the basic medium was supplemented with 0.5 mg/l of 2,4-D, in all tested genotypes. For shoot regeneration, calli were transferred to MS and WPM medium supplemented with BA (1.0-2.5 mg/l) and NAA (0.2 mg/l). Multiple shoot regeneration was observed in each shoot induction medium. The highest rate of shoot regeneration (6.83 shoot/callus) was observed on MS medium containing 2.5 mg/l BA and 0.2 mg/l NAA. The results showed highly significant differences between the media. 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Gergácz</name><name sortKey="Heszky, L E" sort="Heszky, L E" uniqKey="Heszky L" first="L E" last="Heszky">L E Heszky</name><name sortKey="Kiss, J" sort="Kiss, J" uniqKey="Kiss J" first="J" last="Kiss">J. Kiss</name></noCountry><country name="Hongrie"><noRegion><name sortKey="Jafari, M A" sort="Jafari, M A" uniqKey="Jafari M" first="M A" last="Jafari">M A Jafari</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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